Coding

Part:BBa_K4710001:Design

Designed by: Clara Deimling   Group: iGEM23_UChicago   (2023-10-09)


Aromatic Aminoadipate Transaminase (AATA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1012
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized via IDT.

An A->G change was made in two places to remove EcoRI cut sites: A837G, A1233G

Source

The protein sequence was obtained from PDBID 4JE5.

References